Myocardial post-conditioning with Danshen-Gegen decoction protects against isoproterenol-induced myocardial injury via a PKCε/mKATP-mediated pathway in rats
نویسندگان
چکیده
BACKGROUND Danshen-Gegen decoction (DG), a Chinese herbal formula, has been demonstrated to be effective for the treatment of coronary heart disease such as myocardial infarction. In the present study, we investigated the effect of DG post-conditioning on isoproterenol (ISO)-induced myocardial injury in rats. METHODS ISO was injected intraperitoneally (200 mg/kg) to induce acute (2-6 hours) myocardial injury in adult female rats. DG (4 g/kg) was administered per oral immediately after the injection of ISO in the rats. Extent of myocardial injury was assessed by measurements of plasma enzyme activities. Myocardial mitochondrial glutathione antioxidant status, lipid peroxidation and mitochondrial calcium ion loading and cytochrome c release were also measured. Effects of inhibitors of protein kinase C-epsilon (PKCε) ranslocation and mitochondrial ATP-sensitive potassium channel (mKATP) on myocardial post-conditioning by DG were investigated. RESULTS ISO inflicted acute myocardial injury in the rats as evidenced by increased plasma enzyme activities. DG post-treatment alleviated the ISO-induced acute myocardial injury. CONCLUSION DG post-treatment protected the myocardium against ISO-induced acute injury in rats. The myocardial post-conditioning by DG is likely mediated by PKCε/mKATP signaling pathway.
منابع مشابه
Synergestic effect of coenzyme Q10 and magnesium sulphate in reducing myocardial infarction caused by isoproterenol in rats
The objective of the study aims to evaluate the combined protective effects of coenzyme Q10 and magnesium sulphate on isoproterenol induced myocardial damage in rats. CoenzymeQ10 (50 mg/kg) and magnesium sulphate (10 mg/kg) were administered orally to wistar rats in individual or in combination for 30 days. At the end of this period, rats were administered isoproterenol (85 mg/kg i.p.) intrape...
متن کاملEffect of betaine supplement on isoprenaline induced myocardial infarction and serum cathepsin G level in rat model
Background & Aims: Myocardial infarction is one of the most common life threatening diseases in worldwide. Betaine is a safe and well tolerated compound that shows beneficial antioxidant and anti-inflammatory properties. Previous studies demonstrated, betaine reduce cardiovascular diseases but molecular mechanism of action did not known completely. Cathepsin G play pivotal role in tissue injury...
متن کاملAuthor's response to reviews Title: The Cardioprotective Effect of Danshen and Gegen Decoction on Rat Hearts and Cardiomyocytes with Post-ischemia Reperfusion Injury Authors:
متن کامل
Inhibiting miR-155 protects against myocardial ischemia/reperfusion injury via targeted regulation of HIF-1α in rats
Objective(s): The aim of this study was to identify the role of miR-155 in the myocardial ischemia/reperfusion (I/R) injury through targeting hypoxia-inducible factor 1-alpha (HIF-1α). Materials and Methods: We constructed rat models with myocardial I/R injury and H9C2 cell models with hypoxia/reoxygenation (H/R) damage. Anti-miR-155 and...
متن کاملEffects of histidine and vitamin C on isoproterenol-induced acute myocardial infarction in rats
In the present study, we investigated the effects of histidine and vitamin C (alone or in combination) treatments against isoproterenol (a β-adrenergic receptor agonist)-induced acute myocardial infarction in rats. We used propranolol (a β-adrenergic receptor blocker) to compare the results. Rats were given intraperitoneal injections of histidine (40 mg kg-1) and vitamin C (40 mg kg<...
متن کامل